Segway performs semi-automated genome annotation, discovering joint patterns across multiple genomic signal datasets. We discuss a major new version of Segway and highlight its ability to model data with substantially greater accuracy. Major enhancements in Segway 2.0 include the ability to model data with a mixture of Gaussians, enabling capture of arbitrarily complex signal distributions, and minibatch training, leading to better learned parameters.

Availability and implementation

Segway and its source code are freely available for download at We have made available scripts ( and datasets ( for this paper’s analysis.

Supplementary information

Supplementary data are available at Bioinformatics online.

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